NOTE: Masks are required for all guests ages 2 and up, and all member and nonmember tickets must be reserved in advance. Learn more about how you can join us!

#bioPGH Blog: Nature’s Antifreeze
Jan 13

#bioPGH Blog: Nature’s Antifreeze

By Dr. Maria Wheeler-Dubas, Research and Science Education Outreach Manager

Biophilia NetworkA resource of Biophilia: Pittsburgh, #bioPGH is a weekly blog and social media series that aims to encourage both children and adults to reconnect with nature and enjoy what each of our distinctive seasons has to offer. 

Subscribe to Posts Via Email


Though perhaps delayed, winter has finally arrived in Pittsburgh! While many of us snuggle up in blankets on chilly evenings, maybe even by a crackling fireplace, how do plants, animals, even fungi and insects stay alive in freezing weather? Most of our furred and feathered neighbors have insulating layers of, well, fur or feathers to keep warm, and many animals migrate to balmier locations,  but what about the living things with no coats? It turns nature out has a fascinating chemical plan for winter weather management. So let’s combine a little biology with chemistry and explore!

First things first: the real danger of winter for many creatures is not merely freezing: it is the formation of ice crystals. Ice can damage cells by rupturing them or by damaging valuable compounds the cells use on a regular basis. Different organisms have a variety of ways of preparing for freezing conditions, and they often employ multiple tactics. Trees, for instance, drop their leaves in the winter because the energy that would be needed to repair frozen leaf tissue would be more costly than simply growing new leaves in the spring. Of the insects and arachnids who overwinter, many beat the cold by burrowing underground or some other hiding spot that may remain (hopefully) ice free. Those who are waiting for spring often enter a “hibernation” of sorts known as diapause.

In these scenarios, though, the organisms’ actions are coupled with a secret weapon: natural antifreezes. Chemical compounds produced by the organism to either prevent the organism from freezing (freeze-avoiding) or to allow the organism to “safely” freeze (freeze-tolerant). Below are a few of the most common types of natural antifreezes found in our living world. The list is by no means exhaustive, though; part of that is because we simply do not yet know all of the ways that plants and animals can protect themselves from freezing. Are any young biologists up for a challenge?

Sugars—A wide variety of plants, insects, and amphibian species use different combinations of sugars to help prevent freezing. The sugars can lower the freezing point of the fluid in the body, but more importantly, they can help prevent the formation of crystals.  One commonly produced sugar alcohol is glycerol, which is used, for example, by the notorious invasive insect emerald ash borer. Many of our native frogs actually produce glucose as an overwintering antifreeze and trees are well-known for their ability to break down their starch reserves to create sugary antifreeze solutions.

Glycolipids—As their name suggests, these compounds contain a sugar component and lipid (fat) component. Most of the antifreeze properties of glycolipids seem to revolve around the cell membranes, though the exact mechanism is not always clear. Until recently, the use of glycolipids as an antifreeze was most commonly associated with insects, but the protective property has been identified in some plants and at least one frog.

Ice-binding proteins—Perhaps more common in polar species, ice-binding proteins are special compounds produced by the cells of some plants, fungi, insects, and even the occasional algae. Ice-binding proteins work by interrupting the formation of ice crystals and by lowering the freezing point, not unlike road salt; but some ice-binding proteins found in plants are orders of magnitude more effective at manipulating the freezing point than anything we humans have used.

Well now, it might seem easier to make a cup of tea and find a cozy blanket, but nature certainly has a way of making sure even the northernmost of beetles have a way to power through a cold winter!

Connecting to the Outdoors: For days that are too cold to play outside, bring some snowflake magic inside! Check out the video below on snowflake formation, and then try a twist on classic snowflake crafts with an activity below designed by our education facilitator Emily Horner!


For this craft, you will need:

Supplies: A paper towel tube, scissors, a marker, white or blue paint, crafting glue, paper clips or clothespins, yarn or ribbon, and glitter (optional)

Step 1: Find an old paper towel tube, and mark the tube every ½ inch. Flatten the tube out and cut along the lines.

Step 2: Use either white or blue paint to paint the outside and inside of the smaller paper towel tube pieces. I put 2 layers of paint onto each “crystal!”

Step 3: Glue each crystal together into different shapes, and paperclip each crystal together. This will ensure they stick together and the glue dries properly.

Step 4: Let your snowflake sit for an hour or so, and then tie a string onto the snowflake if you would like to hang it.

Make sure to experiment with making different shapes with your snowflakes! Have fun!


Continue the Conversation: Share your nature discoveries with our community by posting to Twitter and Instagram with hashtag #bioPGH, and R.S.V.P. to attend our next Biophilia: Pittsburgh meeting.


Duman 2015: Animal ice-binding (antifreeze) proteins and glycolipids: an overview with emphasis on physiological function

MSU Extension: Winter dormancy and chilling in woody plants

NY Times: When Built-in Antifreeze Beats a Winter Coat

Research Collaboratory for Structural Bioinformatics: Antifreeze Proteins 

Crosthwaite et al. 2011: The overwintering physiology of the emerald ash borer, Agrilus planipennis fairmaire (coleoptera: buprestidae)

Sømme 1982: Supercooling and winter survival in terrestrial arthropods 

Smithsonian Institute: Where Do Insects Go in the Winter?

Gupta and Deswall 2014: Antifreeze proteins enable plants to survive in freezing conditions 

Walters et al 2011: A thermal hysteresis-producing xylomannan glycolipid antifreeze associated with cold tolerance is found in diverse taxa.

Photo credits: Header and cover, both Pexels